SFC 2019 
Short Course Information

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Course Description

This course will focus on fundamentals and advances in supercritical fluid chromatography (SFC) employing carbon dioxide-based mobile phases. The course will also provide an introduction to supercritical fluid extraction (SFE).  Particular emphasis will be directed toward modern instrumentation and method development in both analytical-scale and preparative-scale SFC, including chiral separations.  The advantages of SFC will be illustrated with applications in pharmaceuticals and other fields where SFC currently plays, or can play, a critical role such as lipidomics, specialty chemicals, biodiesel, foods, natural products, polymers, consumer products, and fragrances/flavors.  The course is taught by three scientists (1 academic, 2 industrial) who have spent the majorities of their careers working in SFC and SFE.

Course Outcomes

Course attendees will learn the following:

  • Advantages of SFC over HPLC-based separations and purifications.

  • How to develop analytical SFC separations and preparative SFC purifications.

  • How to choose stationary phases for achiral and chiral SFC separations.

  • How to use SFC to increase the environmental friendliness of separations.

  • Which molecules are suitable for SFC, and which molecules may not be suitable, including a survey of application areas.

  • The roles of mobile phase modifiers and additives in SFC separations.

  • Understanding the roles of pressure and temperature on chromatographic efficiency, selectivity, and phase behavior of the SFC mobile phase.

  • Options for detection in SFC, with special emphasis on SFC/MS.

  • Basic principles and latest developments in SFE.

  • The future of SFC and SFE


Who Should Take This Course

The course is for researchers and managers interested in increasing the efficiency of analyses and separations while reducing costs.

Academic and industrial separation scientists

Process chemists interested in chromatographic purification

Scientists and R&D managers working in the following areas:



Biological sciences

Specialty chemicals

Small polymers 

Consumer products

Advanced materials

Food and beverages

Biodiesel technology

Natural products

Fragrances and flavors


Course Prerequisite

Knowledge of chromatographic principles is desirable. Actual experience or knowledge of SFC or SFE is not required.

SFC Short Course Agenda

September 29,2019
 10:00 a.m.

Introduction of Instructors and Course Orientation

 10:10 a.m.   Introduction to Supercrictical Fluids and pcSFC-Taylor
11:10 a.m. 

Instrumentation in Analytical-Scale SFC-Pinkston

11:40 a.m.

Method Development for Chiral Analytical pcSFC-Miller

12:30 a.m.


1:10 p.m.

Method Development for Achiral pcSFC- Pinkston

2:10 p.m.

Preparative pcSFC-Miller

3:10 p.m.


3:25 p.m.

Detection in Packed Column SFC-Pinkston

4:15 p.m.

Introduction to Supercritical Fluid Extraction-Taylor

5:15 p.m.

Discussion, Q&A

Detailed Course Agenda

Coming Soon.


Biography of Course Instructors


J. David Pinkston is a Principal Scientist in Kellogg’s Global Chemistry organization. His research interests have included the development and application of various forms of pressurized fluid chromatography and the coupling of these separation methods to intelligent detectors, such as mass spectrometry. Most recently, his interests have focused on flavor/aroma chemistry, trace-level contaminants, and oil/fat chemistry, degradation, and preservation. David received his Ph.D. in 1985 from Michigan State University working in separations and mass spectrometry under the direction of J. Throck Watson and John Allison. He was Chair of the ACS’s Division of Analytical Chemistry in 2002 – 2003, was the Division’s Program Chair for the Fall 2002 and Spring 2003 National ACS Meetings, and has served on the Executive Committee of the Subdivision of Chromatography and Separations Chemistry. David has authored or co-authored over 55 publications, and presented over 130 lectures, the majority of which are in the areas of SFC and SFC/MS. He has taught various versions of the SFC short course since the early 1990s. David loves downhill skiing, and is an avid cyclist.

Larry Miller is a Principal Scientist in the Discovery Analytical Sciences group at Amgen in Cambridge, MA.  He graduated with a BS degree from the University of Illinois in Urbana-Champaign and a MS from Roosevelt University. He has over 30 years of experience performing small molecule achiral and chiral purifications at the mg to multi-kg scale.  During his career, he spent 20 years at Searle/Pharmacia and has spent the last 15 years at Amgen   At Amgen he is responsible for discovery and early development purification support utilizing preparative SFC and HPLC.  Larry has over 30 peer reviewed publications, over 40 presentations at scientific meetings and serves as co-instructor for SFC short courses in the US and Europe.  In addition, Larry is the past president of the Green Chemistry Group which organizes the annual SFC conference.

Larry T. Taylor is Emeritus Professor of Chemistry, Virginia Tech (Blacksburg, VA) and President of Applied Analytical, Inc. He is a member of the editorial boards for the Journal of Chromatographic Science, Chromatographia, and the Journal of Supercritical Fluids. He was a member of the organizing committee for the 4th through the 10th International Symposia on SFC/SFE during the 1980s. More recently he has served as the Co-Chair with Larry Miller (Amgen) of the Scientific Committee for SFC 2008 (Zurich), SFC 2009 (Philadelphia), SFC 2010 (Stockholm), SFC 2011 (New York City), SFC 2012 (Brussels), SFC 2013 (Boston), SFC 2014 (Basal), SFC 2015 (Philadelphia), and SFC 2016 (Vienna). Larry is the author or co-author of approximately 400 peer reviewed publications, one book entitled “Analytical Supercritical Fluid Extraction”, and 12 patents. He presently serves as co-teacher of short courses addressed to SFC and SFE. Recent industrial partnerships have included: Waters Corp., Pfizer, Eli Lilly, Princeton Chromatography, ES Industries, Amgen, Applied Separations, Procter & Gamble, Merck, Wythe, and Abbott Labs.